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| Registros recuperados : 7 | |
| 3. |  | OLIVEIRA, A. M. de; DIAS, J. A.; MACEDO, S. C. C.; BELOTI, V.; FARIA, G. V.; PACHECO, I. F. Impacto da adoção de boas práticas na contagem de bactérias mesófilas em pontos de contaminação do leite cru de rebanhos em condições de produção prevalentes em Rondônia. In: ENCONTRO DE INICIAÇÃO À PESQUISA DA EMBRAPA RONDÔNIA, 10.; ENCONTRO DE PÓS-GRADUAÇÃO DA EMBRAPA RONDÔNIA, 5., 2019, Porto Velho. Anais... Porto Velho, RO: Embrapa Rondônia, 2019. p. 26.| Biblioteca(s): Embrapa Rondônia. |
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| 4. | | CONSTANTINO, N. A. S.; DIAS, J. A.; OLIVEIRA, A. M. de; CORDEIRO, S. C. M.; BELOTI, V.; FARIA, G. V. Impacto da adoção de boas práticas na contagem de bactérias mesófilas em rebanhos em condições de produção prevalentes em Rondônia. In: CONGRESSO INTERNACIONAL DE PECUÁRIA LEITEIRA DA AMAZÔNIA OCIDENTAL, 1., 2019, Rio Branco, AC. Anais... Rio Branco, AC: Edufac, 2019. p. 66.| Biblioteca(s): Embrapa Rondônia. |
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| 5. | | CONSTANTINO, N. A. S.; DIAS, J. A.; OLIVEIRA, A. M. de; CORDEIRO, S. C. M.; BELOTI, V.; FARIA, G. V. Pontos críticos de contaminação microbiológica do leite cru nas condições de produção prevalentes em Rondônia. In: CONGRESSO INTERNACIONAL DE PECUÁRIA LEITEIRA DA AMAZÔNIA OCIDENTAL, 1., 2019, Rio Branco, AC. Anais... Rio Branco, AC: Edufac, 2019. p. 75.| Biblioteca(s): Embrapa Rondônia. |
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| 6. | | RIBEIRO JUNIOR, J. C.; TEIDER JUNIOR, P. I.; OLIVEIRA, A. L. M.; RIOS, E. A.; TAMANINI, R.; BELOTI, V. Proteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk. Pesquisa Veterinária Brasileira, Brasília, DF, v. 38, n 8, p. 1577-1583, agosto 2018 Título em português: Proteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 7. | | BELOTI, V.; BARROS, M. de A. F.; AUGUSTO NETO, L.; SANTANA, E. H. W. de; SOUZA, J. A. de; FRANCO, B. D. G. M. Avaliacao do desempenho do ColisureTM na enumeracao de coliformes totais e escherichia coli em leite pasteurizado. Semina, v.21, n.1, p.15-18, 2000.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
18/10/2018 |
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Data da última atualização: |
18/10/2018 |
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Autoria: |
RIBEIRO JUNIOR, J. C.; TEIDER JUNIOR, P. I.; OLIVEIRA, A. L. M.; RIOS, E. A.; TAMANINI, R.; BELOTI, V. |
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Afiliação: |
José C. Ribeiro Júnior; Pedro I. Teider Junior; André L. M. Oliveira; Edson A. Rios; Ronaldo Tamanini; Vanerli Beloti. |
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Título: |
Proteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 38, n 8, p. 1577-1583, agosto 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Proteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk. |
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Conteúdo: |
Pseudomonas, the main genus of gram-negative microorganisms isolated from milk, is psychrotrophic, biofilm-forming, and thermo-resistant deteriorating enzyme producers. The aim of this study was to quantify Pseudomonas spp. in goat?s and cow?s milk produced in the Paraná state, Brazil, to evaluate the deteriorating activity of the isolates at mesophilic and psychrotrophic conditions and to identify, at the species level, the isolates with alkaline metalloprotease (aprX gene) production potential. Microbiological, biochemical and molecular methods were used for isolating, confirming and identifying of isolates. The mean counts were 1.6 (±6.3)x104 and 0.89(±3)x102 CFU/mL for goat and bovine milk samples, respectively, immediately after milking. Of the Pseudomonas colonies isolated from goat milk (n=60), 91.7% showed proteolytic potential when incubated at 35°C/48 h and 80% at 7°C/10 days, and lipolytic potential was observed in 95% of the isolates incubated in mesophilic and 78.3% at refrigeration conditions. From the isolates of bovine milk (n=20), 35% showed proteolytic activity only when incubated at 35°C/48 h, and lipolytic potential was observed in 25% of the isolates incubated at 7°C/10d and 35°C/48h. It was observed that 83.3% and 25% of the isolates genetically confirmed as Pseudomonas spp. of goat and bovine milk showed the potential for alkaline metalloprotease production, with the species P. azotoformans, P. koreensis, P. gessardii, P. monteilii and P. lurida being the most frequent in goat milk and P. aeruginosa the only species identified in cow milk. MenosPseudomonas, the main genus of gram-negative microorganisms isolated from milk, is psychrotrophic, biofilm-forming, and thermo-resistant deteriorating enzyme producers. The aim of this study was to quantify Pseudomonas spp. in goat?s and cow?s milk produced in the Paraná state, Brazil, to evaluate the deteriorating activity of the isolates at mesophilic and psychrotrophic conditions and to identify, at the species level, the isolates with alkaline metalloprotease (aprX gene) production potential. Microbiological, biochemical and molecular methods were used for isolating, confirming and identifying of isolates. The mean counts were 1.6 (±6.3)x104 and 0.89(±3)x102 CFU/mL for goat and bovine milk samples, respectively, immediately after milking. Of the Pseudomonas colonies isolated from goat milk (n=60), 91.7% showed proteolytic potential when incubated at 35°C/48 h and 80% at 7°C/10 days, and lipolytic potential was observed in 95% of the isolates incubated in mesophilic and 78.3% at refrigeration conditions. From the isolates of bovine milk (n=20), 35% showed proteolytic activity only when incubated at 35°C/48 h, and lipolytic potential was observed in 25% of the isolates incubated at 7°C/10d and 35°C/48h. It was observed that 83.3% and 25% of the isolates genetically confirmed as Pseudomonas spp. of goat and bovine milk showed the potential for alkaline metalloprotease production, with the species P. azotoformans, P. koreensis, P. gessardii, P. monteilii and P. lurida being ... Mostrar Tudo |
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Palavras-Chave: |
Alkaline metalloprotease; Bacterioses; Bovine; Deteriorante; Diversidade microbiana; Lipolytic; Microbial diversity; Proteolítico; Pseudomonas spp. |
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Thesagro: |
Bacteriose; Bovino; Caprino; Leite. |
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Thesaurus NAL: |
Goats; Milk; Spoilage. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/184709/1/Proteolytic-and-lipolytic-potential.pdf
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Marc: |
LEADER 02753naa a2200385 a 4500
001 2097757
005 2018-10-18
008 2018 bl uuuu u00u1 u #d
100 1 $aRIBEIRO JUNIOR, J. C.
245 $aProteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Proteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk.
520 $aPseudomonas, the main genus of gram-negative microorganisms isolated from milk, is psychrotrophic, biofilm-forming, and thermo-resistant deteriorating enzyme producers. The aim of this study was to quantify Pseudomonas spp. in goat?s and cow?s milk produced in the Paraná state, Brazil, to evaluate the deteriorating activity of the isolates at mesophilic and psychrotrophic conditions and to identify, at the species level, the isolates with alkaline metalloprotease (aprX gene) production potential. Microbiological, biochemical and molecular methods were used for isolating, confirming and identifying of isolates. The mean counts were 1.6 (±6.3)x104 and 0.89(±3)x102 CFU/mL for goat and bovine milk samples, respectively, immediately after milking. Of the Pseudomonas colonies isolated from goat milk (n=60), 91.7% showed proteolytic potential when incubated at 35°C/48 h and 80% at 7°C/10 days, and lipolytic potential was observed in 95% of the isolates incubated in mesophilic and 78.3% at refrigeration conditions. From the isolates of bovine milk (n=20), 35% showed proteolytic activity only when incubated at 35°C/48 h, and lipolytic potential was observed in 25% of the isolates incubated at 7°C/10d and 35°C/48h. It was observed that 83.3% and 25% of the isolates genetically confirmed as Pseudomonas spp. of goat and bovine milk showed the potential for alkaline metalloprotease production, with the species P. azotoformans, P. koreensis, P. gessardii, P. monteilii and P. lurida being the most frequent in goat milk and P. aeruginosa the only species identified in cow milk.
650 $aGoats
650 $aMilk
650 $aSpoilage
650 $aBacteriose
650 $aBovino
650 $aCaprino
650 $aLeite
653 $aAlkaline metalloprotease
653 $aBacterioses
653 $aBovine
653 $aDeteriorante
653 $aDiversidade microbiana
653 $aLipolytic
653 $aMicrobial diversity
653 $aProteolítico
653 $aPseudomonas spp
700 1 $aTEIDER JUNIOR, P. I.
700 1 $aOLIVEIRA, A. L. M.
700 1 $aRIOS, E. A.
700 1 $aTAMANINI, R.
700 1 $aBELOTI, V.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 38, n 8, p. 1577-1583, agosto 2018
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